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reference strain中文是什么意思

  • 標(biāo)準(zhǔn)物
  • 參考物
  • 參照菌株

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  • 例句與用法
  • The reference strain of bj were clustered into subgroup g independently
    參比菌株bj獨立聚為g亞群。
  • Based on the result of numerical taxonomy , 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains
    一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有專一的酶切圖譜類型。
  • And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual
    G皿z 、 gxnn 、與屬1群的中國主要參考毒株hclv 、 shimen在遺傳關(guān)系上較遠(yuǎn),與italy株和paderborn株比較接近。
  • Substitution and insertion in all strains si gene , the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 %
    S1基因除與qx的親緣關(guān)系較近外,與其它參考株的同源率均低于80 ,可能為一株國內(nèi)流行性的變異株。
  • The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison
    從新分離的和原來保藏的菌株中,選擇了革蘭氏陽性菌株110株和7株參比菌株,進行了菌株表型特性的測定。
  • Results of phenotype test shown that all peanut isolates and reference strains of b . japonicum and b . elkanii were clustered into a group and differed from the other genus of fast - growing rhizobia in low similarity
    表型分析結(jié)果表明所有供試菌株與慢生參比菌株b . japonicum和b . elkanii聚為一群,而其它種屬的參比菌株聚為另一群,表明花生根瘤菌在屬的水平上應(yīng)屬于bradyrhizobium屬。
  • Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels
    為研究病毒的核酸分子結(jié)構(gòu)與其致病性的關(guān)系,本研究選取了在致死率上不同的4個ibdv野毒株,比較了它們的vpz基因高變區(qū)共494個堿基序列。
  • Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali
    本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象,以20株已知種的根瘤菌為參比菌株,采用數(shù)值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現(xiàn)代根瘤菌分類技術(shù),初步研究了葛藤根瘤菌的生物多樣性和分類地位,結(jié)果表明:葛藤根瘤菌在生長速率上表現(xiàn)出多樣性,菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快, yma培養(yǎng)基上28培養(yǎng)2 - 3天后,單個菌落直徑大于1mm ,具有產(chǎn)酸能力,是快生型葛藤根瘤菌;其余待測葛藤根瘤菌生長較慢, yma培養(yǎng)基上28培養(yǎng)7天后,單個菌落直徑小于1mm ,具有產(chǎn)堿能力,是慢生型葛藤根瘤菌。
  • In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
    擴增產(chǎn)物連接到pgem - teasy載體中,轉(zhuǎn)化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質(zhì)粒經(jīng)酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質(zhì)粒pgem - 3abc和表達(dá)載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達(dá)載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發(fā)現(xiàn)克隆到ptriex - 4neo載體上的片段于3abc基因708bp處出現(xiàn)了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導(dǎo)表達(dá),收集菌液進行sds - page電泳、 westernblotting分析,結(jié)果表明, 3ab基因在大腸桿菌中成功表達(dá),其表達(dá)產(chǎn)物為分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒陽性血清識別。經(jīng)薄層掃描分析,表達(dá)量占總蛋白量的26以上。
  • The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years
    結(jié)果發(fā)現(xiàn),廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高,而與其它參考株差異較大;廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特征,并與毒株在臨床上的致病情況相符;根據(jù)apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree )來看,廠西雞和鵝分離株都?xì)w屬于基因型vll 。
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