strain vt. 1.用力拉,拉緊,抽緊,扯緊。 2.使緊張;盡量使用(肌肉等)。 3.強迫,強制;濫用,盡量利用。 4.拉傷,用力過度而弄傷,使工作過度;使用過度而弄壞;扭傷。 5.曲解,牽制附會。 6.【機械工程】使變形,扭歪。 7.抱緊。 8.濾 (out)。 vi. 1.盡力,拼命努力。 2.拉,拖 (at)。 3.扭歪,彎曲,快要折斷。 4.濾過,滲出。 5.不肯接受。 strain one's ears 豎起耳朵注意聽。 strain one's voice 拼命呼喊。 strain one's eyes 睜大眼睛看。 strain one's wit 絞盡腦汁。 strain oneself 過勞。 strain one's authority 濫用權(quán)力。 strain sb.'s good temper 利用某人脾氣好。 Mary strained her baby to the breast. 瑪麗把小孩緊抱在懷里。 strain a rope to the breaking point 將繩拉緊到快要斷的程度。 strained relations between officers and men 搞得不好的官兵關(guān)系。 strain the law 曲解法律。 a strained interpretation 歪曲的翻譯;牽強的解釋。 He is straining under their pressure. 他是在他們的壓力之下苦撐著。 It's the nature of plants to strain upwards to the light. 植物的本性是向上挺竄爭取陽光。 You, too, will strain at such a demand. 你也會難以接受這樣的要求的。 strain a point 逾分,過分,任意(曲解)。 strain after 盡力追求,拼命想得到[做到]。 strain at 為,辛苦[用氣力,費神,盡力]。 strain at a gnat 為小事過分操心。 strain at the oar 拼命劃。 strain courtesy 太講禮貌,過分客氣。 strain every nerve 傾全力;全神灌注 (to do)。 strain under pressure 在壓迫下拼命掙扎。 n. 1.拉緊;緊張;盡力,出力。 2.過勞,使用過度;濫用,利用。 3.扭筋,脫臼。 4.【物理學(xué)】變形,歪曲;應(yīng)力,張力;脅變,應(yīng)變。 5.曲解。 put a great strain on sb.'s resources 使人擔(dān)負(fù)過重的經(jīng)濟負(fù)擔(dān)。 It was a great strain on my resources. 這在我財力上是一個很大的負(fù)擔(dān)。 at full [utmost] strain=be on the strain 緊張,拼命。 stand the strain 拼命忍受。 under the strain 因緊張;因過勞。 n. 1.血統(tǒng),家世;族,種;【生物學(xué)】品系,系;菌株;變種,小種。 2.性格,脾氣;傾向,氣質(zhì)。 3.語氣;筆調(diào),文風(fēng);作風(fēng)。 4.〔常 pl. 〕一段音樂,歌曲;詩歌。 5.一陣子滔滔不絕的言詞;一陣子難聽的話。 She comes of a peasant strain. 她出身于世代農(nóng)家。 The Germany strain in him makes him like philosophy. 他的德意志民族的血統(tǒng)使得他喜歡研究哲學(xué)。 good strains of seed 良種。 a hybrid strain 雜交種。 a meat strain 肉用品種。 He has a strain of melancholy in him. 他有點憂郁。 in the same strain 以同樣調(diào)子[作風(fēng)]。 It was the commencement day and the head master would talk in a lofty strain. 那天是開學(xué)典禮,是校長高談闊論的日子。
reference n. 1.(對委員、審查人等的)委托;委托項目[范圍]。 2.說到,論到,提到。 3.參考;參考書;附注,引證;基準(zhǔn),依據(jù);關(guān)系。 4.(關(guān)于人品、能力等的)查詢,咨詢,詢商;可供查問的人;證明人,介紹人;(身份、能力等的)證明書,介紹書,服務(wù)經(jīng)歷,鑒定書。 5.參看符號〔如 asterisk[*e], obelisk ?, section§, parallel‖, paragraph π等〕 (=reference marks)。 the commission's terms of reference 委員會的職權(quán)。 R- was made to me. 提到了我;所指的是我。 You make no reference to your plan in your letter. 你信里沒有提到你的計劃。 a book of reference=a reference book 參考書。 Who are your references 你的證明人都是誰? bear reference to =have reference to. give a reference to 提到,介紹。 have reference to 和…有關(guān)系。 in reference to 關(guān)于。 load one's pages with references (在著作中)引用大量參考資料[文獻]。 make reference to 說到,涉及,提到;參考;查問;介紹。 with reference to 關(guān)于。 without reference to 不管,不論(without reference to age or sex 無論男女老少)。 vt. 加注,附加注腳;給(書等)列出參考書目[注明資料來源]。
The reference strain of bj were clustered into subgroup g independently 參比菌株bj獨立聚為g亞群。
Based on the result of numerical taxonomy , 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains 一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有專一的酶切圖譜類型。
And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual G皿z 、 gxnn 、與屬1群的中國主要參考毒株hclv 、 shimen在遺傳關(guān)系上較遠(yuǎn),與italy株和paderborn株比較接近。
Substitution and insertion in all strains si gene , the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 % S1基因除與qx的親緣關(guān)系較近外,與其它參考株的同源率均低于80 ,可能為一株國內(nèi)流行性的變異株。
The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison 從新分離的和原來保藏的菌株中,選擇了革蘭氏陽性菌株110株和7株參比菌株,進行了菌株表型特性的測定。
Results of phenotype test shown that all peanut isolates and reference strains of b . japonicum and b . elkanii were clustered into a group and differed from the other genus of fast - growing rhizobia in low similarity 表型分析結(jié)果表明所有供試菌株與慢生參比菌株b . japonicum和b . elkanii聚為一群,而其它種屬的參比菌株聚為另一群,表明花生根瘤菌在屬的水平上應(yīng)屬于bradyrhizobium屬。
Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels 為研究病毒的核酸分子結(jié)構(gòu)與其致病性的關(guān)系,本研究選取了在致死率上不同的4個ibdv野毒株,比較了它們的vpz基因高變區(qū)共494個堿基序列。
Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali 本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象,以20株已知種的根瘤菌為參比菌株,采用數(shù)值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現(xiàn)代根瘤菌分類技術(shù),初步研究了葛藤根瘤菌的生物多樣性和分類地位,結(jié)果表明:葛藤根瘤菌在生長速率上表現(xiàn)出多樣性,菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快, yma培養(yǎng)基上28培養(yǎng)2 - 3天后,單個菌落直徑大于1mm ,具有產(chǎn)酸能力,是快生型葛藤根瘤菌;其余待測葛藤根瘤菌生長較慢, yma培養(yǎng)基上28培養(yǎng)7天后,單個菌落直徑小于1mm ,具有產(chǎn)堿能力,是慢生型葛藤根瘤菌。
In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis 擴增產(chǎn)物連接到pgem - teasy載體中,轉(zhuǎn)化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質(zhì)粒經(jīng)酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質(zhì)粒pgem - 3abc和表達(dá)載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達(dá)載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發(fā)現(xiàn)克隆到ptriex - 4neo載體上的片段于3abc基因708bp處出現(xiàn)了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導(dǎo)表達(dá),收集菌液進行sds - page電泳、 westernblotting分析,結(jié)果表明, 3ab基因在大腸桿菌中成功表達(dá),其表達(dá)產(chǎn)物為分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒陽性血清識別。經(jīng)薄層掃描分析,表達(dá)量占總蛋白量的26以上。
The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years 結(jié)果發(fā)現(xiàn),廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高,而與其它參考株差異較大;廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特征,并與毒株在臨床上的致病情況相符;根據(jù)apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree )來看,廠西雞和鵝分離株都?xì)w屬于基因型vll 。